One-Step RT PCR Kit

One-Step RT PCR Kit is designed for a Highly Efficient cDNA Synthesis and PCR in a single tube. The kit includes a PCR MasterMix supplied in a 2x concentration to perform standard PCR. MasterMix contains all the reagents needed for running PCR reactions (except PCR primers and template). In addition, a separate RT Mix composed by a balanced mixture of both RTase and RNase Inhibitor is also provided. RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the Reverse Transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step synthesis, Taq DNA Polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA Polymerase exponentially amplifies the double-stranded DNA template.

Includes for 100 rxn:
– 2 x 1. 25 mL One-Step PCR Mix (2x) – 2 x 125 μL RT Mix – 2 mL Water (RNase-free)

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