Name: Oligomycin A
Price: 285.00 USD

- Overview
  - Oligomycin A is a dominant analog of the Oligomycin class of macrocyclic lactones isolated from selected strains of Streptomyces sp. The class includes the analogs/isomers A through G. Different isomers are highly specific for the disruption of mitochondrial metabolism. Oligomycin A exhibits a broad biological profile including antifungal, antitumor and nematocidal activities.
    Oligomycin A is an inhibitor of mitochondrial F1F0 ATP synthase. The Oligomycin complex was first reported in 1954, from a strain of Streptomyces diastatochromogenes from soil which was highly active against fungi. Oligomycins exhibit apoptotic cytotoxicity and mitochondrial toxicity.
    Oligomycin A is soluble in ethanol, methanol, DMSO and DMF. It has poor water solubility.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Oligomycin inhibits phosphoryl group transfer in mitochondrial membrane-bound ATP synthase (F1F0 ATPase), blocking proton translocation and leading to hyperpolarization of inner mitochondrial membrane. The result is that mitochondrial ATP is not synthesized.
    After more than 50 years of studies on the binding site of Oligomycin, a team at the Rosalind Franklin University (North Chicago, IL) discovered that it binds to the subunit-c of the F0 portion of the ATP synthase. The residues involved in the binding site are conserved from yeast to humans (Symersky et al, 2012).
- Properties
  - CAS Number
    
    579-13-5
    
    Molecular Formula
    
    C45H74O11
    
    Molecular Weight
    
    791.1
    
    Appearance
    
    White lyophilisate
    
    Solubility
    
    Oligomycin A is soluble in ethanol, methanol, DMSO and DMF. It has poor water solubility.
    
    Other Properties
    
    Source: Streptomyces diastatochromogenes
    Purity Level: >95% by HPLC
    
    Storage
    
    -20°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Oligomycin A is active against fungi including Aspergillus and Penicillium.
    
    Microbiology Applications: A number of mutations in yeast have been shown to confer resistance to Oligomycin.
    
    Eukaryotic Cell Culture Applications: Oligomycin is used to study ATP-linked respiration and is used for the maximal capacity respiration assay in astrocytes, at 1 µM for MEFs and 2 µM for astrocytes. It can be used to measure oxygen consumption in commercial kits such as the Mito Stress Test kit (Agilent). The compound is serially injected to measure ATP-linked respiration. The test can help quantify parameters of mitochondrial respiration and identify mitochondrial dysfunction (Atzmon et al, 201). Oligomycin can be used to measure oxygen consumption rate and extracellular acidification rates in breast cancer cell lines, contributing to our understanding of molecular pathways that contribute to breast cancer progression (Furth et al, 2018).
    
    Cancer Applications: Mitochondria are regulators in apotosis, thus are a target for cancer research. Oligomycin was found to bypass doxorubicin resistance and block P-glycoprotein activity. P-glycoprotein causes multidrug resistance, and extrudes anticancer drugs to the extracellular environment using ATP. The result was that it triggered apoptosis in drug-resistant HepG2 cells (Li et al, 2002).
    Oligomycin has been used to study the mechanistic aspects of ATP formation in tumor cell biology and apoptosis.

Datasheet

We Also Recommend

21-Hydroxyoligomycin A

$416

Oligomycin B

$285

Oligomycin B

$287

Oligomycin C

$416

Oligomycin C

$602

Oligomycin D

$402

Oligomycin E

$402

Oligomycin A

Oligomycin A

Background

We Also Recommend