Nitro-PAPS

- Overview
  - Nitro-PAPS is a highly sensitive colorimetric reagent for Fe(II) detection that forms a water-soluble complex at pH 3.0-8.0 (λmax=582 nm, λ=107,000). This reagent is suitable for the determination of Fe(II) in serum and can also be used to determine micromolar levels of Cu, Zn, Ni, and Co. Cu and Zn in serum are masked by a mixture of thioglycolic acid and SDS. Zn in serum can be determined by Nitro-PAPS using CN- as a masking reagent of Fe and Cu.
    
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- Properties
  - Alternative Name
    
    2-(5-Nitro-2-pyridylazo)-5-[N-n-propyl-N-(3-sulfopropyl)amino]phenol, disodium salt, dihydrate
    
    CAS Number
    
    143205-66-7 (anhydrous)
    
    Molecular Formula
    
    C17H19N5Na2O6S, 2H2O
    
    Molecular Weight
    
    503.45
    
    Appearance
    
    Dark green or dark greenish-brown powder
    
    Purity
    
    ≥90.0% (anhydrous)
    
    Storage
    
    ambient temperature, protect from light and moisture
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Reference
  - 1) T. Makino, et al., A Sensitive, Direct Colorimetric Assay of Serum Iron Using the Chromogen, Nitro-PAPS. Clin Chim Acta. 1988;171:19-27.
    
    2) T. Makino, A Sensitive, Direct Colorimetric Assay of Serum Zinc Using Nitro-PAPS and Microwell Plates. Clin Chim Acta. 1991;197:209-220.
    
    3) S. Yamashita, et al., Sensitive, Direct Procedures for Simultaneous Determination of Iron and Copper in Serum, with Use of 2-(5-Nitro-2-pyridylazo)-5-(N-propyl-N-sulfopropylamino)phenol(Nitro-PAPS) as Ligand. Clin Chem. 1992;38:1373-1375.
    
    4) T. Yamane, et al., Flow-injection Spectrophotometric Determination of Trace Iron in Various Salts. Elimination of Blank Peak Effect and Use of2-(5-Nitro-2-pyridylazo)-5-(N-propyl-N-sulfopropylamino)phenol as Chromogenic Agent. Anal Chim Acta. 1995;308:433-438.
    
    5) T. Yamane, et al., Complex Formation of 2-(5-Nitro-2-pyridylazo)-5-(N-propyl-N-sulfopropylamino)phenol with Lead, Cadmium and Manganese for Their Sensitive Spectrophotometric Detection in Flow Injection and Ion Chromatography Systems. Anal Chim Acta. 1997;345:139-146.

MSDS

Nitro-PAPS

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