Nitric Oxide (NO) Assay (Colorimetric)

Nitric oxide (NO) is a free radical and signaling molecule that plays an important role in vasodilation, immune system function, apoptosis and many other physiological and pathological processes. Nitric oxide is generated either via the oxidation of L-arginine by NO synthases (NOSs) or the reduction of dietary Nitrite (NO2) and Nitrate (NO3) by various metal-containing proteins. Nitric oxide is an unstable molecule that quickly degrades, which makes it challenging for direct detection methods. However, as the NO2 and NO3 are the stable metabolites of NO oxidation, their concentration is conveniently measured via colorimetric methods and reported as the total NO content in biological samples.

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