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All lanes : NIH/3T3 Whole Cell Lysate (40ug). Lane 1: Anti-GAPDH antibody (ATGA0181). Lane 2: Anti-Beta tubulin antibody (ATGA0196)

All lanes : NIH/3T3 Whole Cell Lysate (40ug). Lane 1: Anti-GAPDH antibody (ATGA0181). Lane 2: Anti-Beta tubulin antibody (ATGA0196)

NIH/3T3 Cell Lysate

NIH/3T3 Cell Lysate

Catalog Number:

CL01443493NKM

Mfr. No.:

ATGL0017

Price:

$260

Size:

Quantity:

Add to Cart:

- Overview
  - NIH/3T3 cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Cell Lysates
    
    Formulation
    
    Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
    
    Concentration
    
    2mg/ml (determined by BCA assay)
    
    Other Properties
    
    Quality Control: Western Blot analyzed by both GAPDH and Beta tubulin antibody
    
    Storage
    
    Aliquot the lysate into single tube and then storing them -80°C or below. Avoid repeated freezing and thawing cycles.
    
    * For research use only. Not intended for human, diagnostics or veterinary use.
- Applications
  - Application Description
    
    Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

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