NHS-biotin

NHS-biotin, product number 10205, is a classic, non-dPEG® biotinylation reagent. Biotin (5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid, CAS number 58-85-5) is a water-soluble vitamin. A wide range of biotechnology and clinical applications use biotin. NHS-biotin (2,5-dioxopyrrolidin-1-yl 5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate, CAS number 35013-72-0) is the N-hydroxysuccinimide ester of biotin. The NHS ester is reactive with free primary amines. NHS-biotin is useful for labeling proteins, peptides, surfaces (such as silica, glass, or magnetic particles) coated with primary amines, and the like. It can also be used with amine-terminated dPEG® products to create novel biotinylated dPEG® products.


NHS-biotin, product number 10205, is not soluble in water, but the NHS ester will hydrolyze if the product gets wet. The optimal storage temperature of NHS-biotin is -20°C. When removed from storage, PN10205 should be allowed to equilibrate fully to ambient temperature before opening the bottle. The product always should be handled to minimize exposure to moisture. NHS-biotin dissolves in N,N'-dimethylacetamide (DMAC) or dimethylsulfoxide (DMSO). DMAC and DMSO must be dried over 3Å molecular sieves for at least 24 hours before use. Solutions of NHS-biotin must be used immediately. The storage of NHS-biotin in solution is not recommended.

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