Name: Natural Der f 1 Molecular Reference Standard (MRS-NDF1)
Price: 925.00 USD

- Overview
  - The Natural Der f 1 MRS is intended to serve as reference standard to determine the Der f 1 content of allergen preparations from house dust mite(Dermatophagoides farinae) by immunoassay
    
    Molecular Weight: 24 kD
    Composition: 10µg natural Der f 1, determined by Amino Acid Analysis, freeze dried in sealed glass vial.
    SDS-PAGE: See inset. Silver-stained SDS-PAGE under non-reducing conditions shows a single band at 24 kD.
    ELISA: Immunoreactive in Der f 1 specific ELISA. No trace contamination with Der f 2 was detected by ELISA.
    
    Please contact us at for specific academic pricing.
- Properties
  - Formulation
    
    Prior to lyophilization, natural Der f 1 was adjusted to 50 mM volatile ammonium bicarbonate with 3% trehalose.
    
    Storage
    
    Store at -20°C
    
    * Allergens are provided for research and commercial use in vitro. Not for human in vivo or therapeutic use.
- Applications
  - Application Description
    
    Reconstitution:
    • Allow vial to reach room temperature before use
    • Tap vial gently to collect all material at the bottom
    • Using a sterile syringe reconstitute the MRS to desired concentration by injecting a suitable volume of a buffer of choice (e.g. PBS, pH 7.4 or 1% BSA/50% glycerol/PBS, pH 7.4).
    • Mix by gently swirling the vial until content is completely dissolved.
    • Adding 1ml of buffer will result in a Der f 1 concentration of 10,000ng/ml.
- Reference
  - Lombardero M et al. Conformational stability of B cell epitopes on Group I and Group II Dermatophagoides spp. allergens. Effect of thermal and chemical denaturation on the binding of murine IgG and human IgE antibodies. J Immunol 1990; 144:1353-1360.
    Schultz O et al. A sensitive fluorescent assay for measuring the cysteine protease activity of Der p 1, a major allergen from the dust mite Dermataphagoides pteronyssinus. J Clin Pathol: Mol Pathol 1998;51:222-231.
    Chapman MD et al. The European Union CREATE project: a model for international standardization of allergy diagnostics and vaccines. J Allergy Clin Immunol. 2008;122:882-889.
    van Ree R et al. The CREATE project: development of certified reference materials for allergenic products and validation of methods for their quantification. Allergy. 2008;63(3):310-26.
    Kaul S et al. Regulatory environment for allergen-specific immunotherapy. Allergy 2011;66:753-64.
    Chapman MD and Briza P. Molecular approaches to allergen standardization. Curr Allergy Asthma Rep. 2012;12:478-84.
    Chapman MD et al. Technological Innovations for High-Throughput Approaches to In Vitro Allergy Diagnosis. Curr Allergy Asthma Rep. 2015;15:36.

Natural Der f 1 Molecular Reference Standard (MRS-NDF1)

Natural Der f 1 Molecular Reference Standard (MRS-NDF1)

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