NADP+ & NADPH Kit (RUO), Quantitative Assay Kit for Whole Blood

Key Features
· Highly accurate and sensitive.
· Easy to handle. One step metabolite extraction is followed by separate measurement of NADP+ and NADPH in the extract. Detection time is less than 10 minutes.
· High throughput. Can be easily adopted into high-throughput 96-well plate assay to analyze hundreds of samples per day.

Principals
The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NADP+ and NADPH metabolites are extracted together from a blood sample in a single step. Then the extract is divided into two parts revealing both NAPD+ and NADPH concentrations by removing one metabolite and stabilizing the other.

Reagents Provided
Extraction BUFFER A, NAPD+ stabilizing reagent, NADPH stabilizing reagent, NADP+ standard stock, NADPH standard stock, Assay BUFFER C, Assay color reagent, Enzyme, Stop solution, Positive control

Kit Requires
Multi-channel pipets. Two clear-bottom 96-well plates suitable for colorimetric assays, heat block with adjustable temperature (up to 80°C), spectrophotometric plate reader

Precautions and Warnings
· The Stop Solution may cause skin, eye and respiratory irritation. Avoid breathing in fumes.
· Assay color reagent may cause skin irritation. Handle with care, use gloves.
· BUFFER A can cause eye irritation. Handle with care, use googles.

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