MycoSPY® qPCR Master Mix - Real-Time Mycoplasma Test Kit

PCR test kit for rapid, highly sensitive detection of mycoplasmas in cell cultures

Highlights:
• Detects all cell-culture-relevant mycoplasma strains
• Highly sensitive
• Master Mix with FAM/HEX fluorescence probes allows for easiest protocol (see workflow)
• Fast results in less than 1.5 hours without the need for gel electrophoresis.

A number of options are available for conclusive detection of the presence of mycoplasmas in contaminated cell cultures. A commonly but unreliable method is the DAPI staining of cells followed by microscopic analysis. However, PCR based methods give the most reliable results for routine checks. MycoSPY®, MycoSPY® Master Mix and MycoSPY® qPCR Master Mix detection kits are rapid, reliable and highly sensitive methods of PCR-based mycoplasma detection in cell cultures.

The MycoSPY® qPCR Master Mix consists of all necessary components for PCR analysis (hot-start Taq polymerase, buffer, dNTPs, primer, FAM/HEX fluorescence probes). The kit includes an Internal Control and PCR-grade water for rehydrating the lyophilized master mix. With a real-time PCR thermocycler suitable for detecting FAM and HEX fluorescent dyes, mycoplasma can be detected quickly and easily in cell cultures.

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