mTOR Phospho Antibody Array

mTOR Antibody Array features 138 highly specific antibodies related to the mTOR pathway. The array is designed for protein phosphorylation profiling in cell and tissue samples from human, mouse, and rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

The mechanistic target of rapamycin (mTOR) is a serine-threonine protein kinase that regulates cell growth, cell proliferation, protein synthesis, and autophagy. Abnormal mTOR signaling has been associated with neurological disorders, cancer, and diabetes. mTOR forms two distinct complexes: mTOR Complex 1 (mTORC1) and mTOR Complex 2 (mTORC2). mTOR binds with the regulatory protein raptor and other binding proteins to form mTORC1 while it binds with regulatory protein rictor and other proteins to form mTORC2. mTORC1 phosphorylates p70S6K and eIF4E to promote mRNA translation and protein synthesis. Growth factors such as insulin trigger the AKT pathway, which signals downstream to mTORC1 to promote cell growth and inhibit autophagy. Oppositely, nutrient deprivation and low energy levels activate the AMPK pathway, which inhibits mTORC1, thus restricting cell growth and promoting autophagy. mTORC2 can affect metabolism and cell survival through phosphorylation of AKT. mTORC2 regulates the cytoskeleton through PKC alpha activation and also influences ion transport.

mTOR Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It is suitable for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine, serine, and threonine phosphorylation at specific sites.

Key Features:
· Site-specific phosphorylation profiling and screening with 138 antibodies
· Antibodies covalently immobilized on 3D polymer coated glass slide
· Fluorescent detection

Specifications:
· Product Size: 2 array slides per package for two samples (untreated vs. treated)
· Featured Antibodies: 138 site-specific and phospho-specific antibodies; 6 replicates per antibody
· Reactivity: Human: 100% | Mouse: 97% | Rat: 83%
· Suitable Sample Type: Cell lysate | Tissue lysate
· Detection Method: Fluorescence | Compatible Scanners
· Internal Controls: Positive controls: beta-actin | GAPDH | Negative controls
· Slide Dimensions: 76 x 25 x 1 mm

Target Proteins:
4E-BP1 (Ser65), 4E-BP1 (Thr36), 4E-BP1 (Thr45), 4E-BP1 (Thr70), AKT1 (Ser124), AKT1 (Ser246), AKT1 (Thr450), AKT1 (Thr72), AKT1 (Tyr474), AKT1S1 (Thr246), AKT2 (Ser474), AKT (Ser473), AKT (Thr308), AKT (Tyr326), AMPK1/AMPK2 (Ser485/491), AMPK1 (Thr174), AMPKbeta1 (Ser182), BAD (Ser112), BAD (Ser134), BAD (Ser136), BAD (Ser155), BAD (Ser91/128), eIF2A (Ser51), eIF4B (Ser422), eIF4E (Ser209), eIF4G (Ser1108), ERK1/2, ERK1 (Thr202), ERK1 (Tyr204), ERK3 (Ser18), ERK8 (Thr175/Tyr177), GSK3a (Ser21), GSK3a-b (Tyr216/270), GSK3b (Ser9), IR (Tyr1355), IR (Tyr1361), Mnk1 (Thr385), mTOR (Ser2448), mTOR (Ser2481), mTOR (Thr2446), P70S6K beta (Ser423), P70S6K (Ser371), P70S6K (Ser411), P70S6K (Ser418), P70S6K (Ser424), P70S6K (Thr229), P70S6K (Thr389), P70S6K (Thr421), P90RSK (Ser380), P90RSK (Thr359/Ser363), P90RSK (Thr573), PDK1 (Ser241), PFKFB2 (Ser483), PI3K-p85-a (Tyr607), PI3K-p85-a/g (Tyr467/Tyr199), PIP5K (Ser307), PKC alpha (Tyr657), PKC alpha/beta II (Thr638), PP2A-alpha (Tyr307), PPAR-b (Thr1457), PPAR-g (Ser112), PTEN (Ser370), PTEN (Ser380), PTEN (Ser380/Thr382/Thr383), Rho/Rac GEF2 (Ser885), RSK1/2/3/4 (Ser221/227/218/232), Tuberin (Ser939), Tuberin (Thr1462)

Assay Outline:
The ELISA based mTOR Pathway Phospho Antibody Array platform involves four major steps:
· Protein extraction with non-denaturing lysis buffer
· Biotinylation of protein samples
· Incubation of labeled samples with antibody array
· Detection by dye conjugated streptavidin

Please contact us at for specific academic pricing.

Background

· High-Throughput Antibody Arrays
Our antibody arrays provide a high-throughput, ELISA-based solution for protein expression profiling across samples like cells, tissues, serum, and culture media. It offers greater productivity and cost savings compared to traditional Western Blots or ELISA.

· Comprehensive Protein Profiling
Choose from our comprehensive arrays: Phospho Explorer Array, Signaling Explorer Array, and Explorer Antibody Array. Profile up to 1,358 biomarkers on a single slide, with options tailored to specific pathways in immunology, oncology, and cell biology.

· Phosphorylation Profiling
Our phosphorylation profiling antibody arrays include the Phospho Explorer Array with 1318 antibodies and Pathway Phospho Arrays covering more than 25 different pathways. This platform features phospho-specific antibodies and paired site-specific antibodies for total protein. It is ideal for tyrosine, serine, and threonine phosphorylation analysis.

· High-Quality, Reliable Arrays
Our antibody arrays feature antibodies covalently attached to premium glass with a 3D polymer coating for optimal binding and specificity. Each includes positive/negative controls and multiple replicates for accurate, reliable results.