Mouse / Rat Obestatin ELISA

Obestatin is a 23 amino acid residue peptide isolated from the rat stomach. The peptide shares its precursor with a food intake stimulating peptide, ghrelin, but possesses reducing effects on food intake, gut motility, and body weight.
With the use of an antiserum directed against the mouse/rat obestatin, obestatin immunoreactivity (irOBS) was detected in cells of the gastric mucosa and myenteric plexus, and in Leydig cells of the testis in Sprague–Dawley rats. Double labeling of myenteric plexus with antisera against obestatin and choline acetyltransferase (ChAT) revealed that nearly all irOBS neurons were ChAT positive and vice versa. Obestatin (100 nM) added to dissociated and cultured rat cerebral cortical neurons elevated cytosolic calcium concentrations, [Ca+2], in a population of cortical neurons. Intracerebroventricular administration of obestatin inhibited water drinking in ad libitum fed and watered rats and in food and water deprived animals. In addition, obestatin inhibited angiotensin II-induced water drinking in animals provided free access to water and food . Obestatin peptides had no effect on insulin sensitivity as revealed by hypoglycemic response when co-administered with insulin, supporting the role of obestatin in regulating metabolism through changes of appetite, but indicating no direct actions on glucose homeostasis or insulin secretion. It is supposed that in rats the effects of obestatin on food intake may be secondary to an action of the peptide to inhibit water drinking .
The Mouse/Rat Obestatin ELISA is for Research Use Only. The Mouse/Rat Obestatin ELISA is not for use in diagnostic procedures.

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