MMT-Amino C-12 CED phosphoramidite

Amino Modifiers

C41H60N3O3P

673.910

This material is sensitive to heat and moisture. It requires the fastest shipment possible.

MMTr-Amino C6 & C12 Modifiers:

Amidites with C6- (MMTr-amino-C6-phosphoramidite, Catalog # CLP-1563) and C12 Spacer (MMTr-amino-C12 phosphora­midite, Catalog # CLP-1585) are used to introduce a primary amino group at the 5’-terminal of an oligonucleotide.
The MMTr on the amino function is cleaved with 3% trichloroacetic acid (TCA), similar to DMTr removal in oligonucleotide synthesis. However, the MMTr-group removal is significantly slower (12 minutes contact with TCA).
A useful utilization of MMTr protected C6 and C12 phosphorami­dites involve the selective cleavage of the MMTr group from the synthesized oligonucleotide to obtain the free terminal amino function while the oligo is still bound to the solid support.
Various chromo­phores and biologically significant molecules can be coupled to the terminal amino group of the support bound oligonucleotide. As it is a solid phase synthesis, excess reagent (chromophores) can easily be washed off, thus leaving almost pure chromophore-oligonucleotide conjugates.