Mixed Cortical Neurons - Human iPSC-Derived

Origin: Human iPSC line

Contents: 1 vial of Cortical Glutamatergic Neurons (>5M Viable Cells), 1 vial of Cortical GABAergic Neurons (>1M Viable Cells), and supplements

Cryopreserved

Marker Expression
Mixed Cortical Neurons (BX-0500) include separate vials cortical glutamtergic neurons and cortical GABAergic neurons, allowing the user to choose their desired ratio of excitatory and inhibitory neurons. The vialed cells have high neuronal purity (>90%) and we recommend mixing at a 5:1 ratio. Labeling with the pan-neuronal marker MAP2 (green), the glutamatergic-specific marker CTIP2 (purple), and GABA (red) highlight the purity and composition of this neuronal product.

Morphology
Mixed Cortical Neurons (BX-0500) are adherent cells and exhibit substantial neurite outgrowth within a week in culture. Calcein staining (green) demonstrates the characteristic cell shape and extension of processes of cortical neurons in culture.

Function
Mixed Cortical Neurons (BX-0500) exhibit pronounced electrophysiological activity after two weeks in culture, as demonstrated by multi-electrode array (MEA) recordings.

Calcium Influx Assays: Changes in calcium concentration are closely tied to neuronal activity as action potentials are associated with large pre-synaptic calcium influx and a notable rise in postsynaptic calcium at excitatory synapses. This can be observed experimentally by stimulating the neurons or culturing the neurons under suitable conditions to form mature networks that exhibit spontaneous oscillations. The influx of calcium can be measured using a variety of calcium-sensitive fluorescent dyes, which are commercially available.

MEA Assays: Multi-electrode arrays (MEA) measure extracellular voltage changes that occur as neurons fire action potentials. These measurements reveal the firing patterns of individual neurons as well as the patterns of neuronal networks that exist in the cell culture. Such measurements are non-invasive and allow for repeated recordings.

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