Name: MitoStep + Annexin V Apoptosis Detection Kit with 7-AAD
Price: 655.00 USD

- Overview
  - Membrane potential is generated and maintained by concentration gradients of ions such as sodium, potassium, chloride, and hydrogen. Mitochondrial drives the accumulation in mitochondria of cationic dyes such as cyanines, and the mitochondrial is reduced when energy metabolism is disrupted, notably in apoptosis. Changes in the mitochondrial potential have been described during necrosis, cell cycle and apoptosis. Mitochondrial uptake of dye is a possible source of fluorescence variance. Flow cytometry can be used to estimate membrane potential in eukaryotic cells. Methods using cyanines dyes can detect changes in membrane potential. Immunostep MitoStep uses a cationic dye DilC1(5) (1,1)Aplication Reference|
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Apoptosis Detection
    
    Alternative Name
    
    Annexin A5
    
    Molecular Weight
    
    35.9 kDa
    
    Other Properties
    
    Conjugated: DilC1(5) + PE
    Gene ID: 308
    
    Storage
    
    DilC1(5), 500 μlof 10μM in DMSO + The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3). Store in the dark at 2-8 °C.
    
    Regulatory Status
    
    RUO
    
    * For research use only.
- Applications
  - Application
    
    Flow Cytometry
    
    Application Description
    
    This reagent is intended for RUO in the identification of apoptotic cells. For flow cytometric staining, the suggested use of this reagent is 5 µl per 1 - million cells in a 100 µl volume Buffer

Datasheet

MSDS

MitoStep + Annexin V Apoptosis Detection Kit with 7-AAD

MitoStep + Annexin V Apoptosis Detection Kit with 7-AAD

We Also Recommend

MitoStep (Mitochondrial Membrane Potencial Assay)