Name: Microbial transglutaminase with C-terminal His6-Tag
Price: 819.00 USD

- Overview
  - Transglutaminases are a family of enzymes that catalyze the posttranslational modification of proteins by inserting an isopeptide bond within or between polypeptide chains. These enzymes catalyze the acyl transfer reaction between the g-carboxyamide groups of peptide-bound glutamine residues and a variety of primary amines, particularly the e-amino group of lysine. The resulting crosslink is of great significance since it is highly stable and also resistant to mechanical and proteolytic degradation.
    
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- Properties
  - Name
    
    EC 2.3.2.13; Protein-glutamine-g-glutamyltransferase
    
    Source
    
    E. coli
    
    Purification
    
    >95 % (214 nm) by HPLC
    
    Formulation
    
    The purified transglutaminase is lyophilized from 50 mM HEPES pH 7.4.
    
    Storage
    
    Store at -80°C. If storage at -80°C is not possible, storage at ≤ -20°C is recommended. While no formal stability data are available at -20°C, according to our overall experience stability is still given. Upon reconstitution, store undiluted working aliquots preferably at -80°C (if not possible at -20°C, see comment above). Repeated freezing and thawing is not recommended.
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Molecular Weight
    
    9 kDa (activated MTG with N-terminal sequence: FRAPDSDDR...)
    
    Reconstitution
    
    Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves.
    Reconstituted MTG solution might be turbid. However, MTG activity is not affected. In this case, centrifuge the solution and collect the supernatant.
    After reconstitution, the solution should be stored frozen in working aliquots.
    
    Activity
    
    > 25 U/mg [One unit will catalyse the formation of 1 μmole of hydroxamate per min from Z-Gln-Gly-OH and hydroxylamine at pH 6.0 at 37°C, Grossowicz et al. (1950)]
- Applications
  - Application Description
    
    Labeling, immobilisation, conjugation and modification of proteins.

Datasheet

MSDS

Microbial transglutaminase with C-terminal His6-Tag

Microbial transglutaminase with C-terminal His6-Tag

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