MCF-7 Cell Lysate

- Overview
  - MCF-7 cells are breast cancer cell line isolated in 1970 from a 69 year old woman, in 1970s. MCF-7 cells are a well characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin, endothelin, GFAP, neurofilament, vimentin.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Cell Lysates
    
    Formulation
    
    Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
    
    Concentration
    
    2mg/ml (determined by BCA assay)
    
    Other Properties
    
    Quality Control: Western Blot analyzed by both GAPDH and Beta tubulin antibody
    
    Storage
    
    Aliquot the lysate into single tube and then storing them -80°C or below. Avoid repeated freezing and thawing cycles.
    
    * For research use only. Not intended for human, diagnostics or veterinary use.
- Applications
  - Application Description
    
    Heat lysate to 95°C for 5 minutes and rapidly cooling before use.