Name: MBP (Maltose Binding Protein) - ²H¹³C¹⁵N
Price: Inquiry

- Overview
  - MW = 40.3 kDa calculated. Recombinant Maltose binding protein from E. coli, expressed in E. coli. The protein consists of the residues 27-392 with a mutation the T28I (UniProtKB accession P0AEX9).
    
    · Characteristics
    Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated up to 1.5 mM.
    
    Labeling: 2H13C15N
    Physical state: Solution
    NMR Standard: Bulk sample
    Shipping in Dry Ice: no
    
    Please contact us at for specific academic pricing.
- Properties
  - Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating between 35.0 and 45.0 kDa.
    
    Formulation
    
    1.0 mg/mL solution in phosphate buffer 20 mM pH 7.2, EDTA 0.1 mM, NaN3 3 mM, Maltotriose 3 mM, Pefabloc® (AEBSF) 50 uM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 66350 M^-1cm^-1 calculated).
    (Unless otherwise specified, NMR tubes will be sold at a concentration of 1mM)
    
    Storage
    
    -20°C. The protein is stable at 4°C for months.
    Avoid repeated freeze/thaw cycles.
    
    Molecular Weight
    
    40.3 kDa
    
    Expression system
    
    E. coli
    
    Sequence
    
    30 40 50
    M-KIEE GKLVIWINGD KGYNGLAEVG
    60 70 80 90 100
    KKFEKDTGIK VTVEHPDKLE EKFPQVAATG DGPDIIFWAH DRFGGYAQSG
    110 120 130 140 150
    LLAEITPDKA FQDKLYPFTW DAVRYNGKLI AYPIAVEALS LIYNKDLLPN
    160 170 180 190 200
    PPKTWEEIPA LDKELKAKGK SALMFNLQEP YFTWPLIAAD GGYAFKYENG
    210 220 230 240 250
    KYDIKDVGVD NAGAKAGLTF LVDLIKNKHM NADTDYSIAE AAFNKGETAM
    260 270 280 290 300
    TINGPWAWSN IDTSKVNYGV TVLPTFKGQP SKPFVGVLSA GINAASPNKE
    310 320 330 340 350
    LAKEFLENYL LTDEGLEAVN KDKPLGAVAL KSYEEELAKD PRIAATMENA
    360 370 380 390
    QKGEIMPNIP QMSAFWYAVR TAVINAASGR QTVDEALKDA QT
    
    * This product is intended for research use or further manufacturing only.
- Reference
  - Ligand-induced structural changes to maltodextrin-binding protein as studied by solution NMR spectroscopy

MBP (Maltose Binding Protein) - ²H¹³C¹⁵N

MBP (Maltose Binding Protein) - ²H¹³C¹⁵N

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