MAL-dPEG®₂₄-TFP ester

MAL-dPEG®24-TFP ester, product number 10554, is a crosslinking reagent with a polyethylene glycol (PEG) cross-bridge that joins free amines to free thiols (sulfhydryl groups). The sulfhydryl groups react with a maleimido group via a Michael addition reaction. The amines form amide bonds with the crosslinker by nucleophilic substitution of the 2,3,5,6-tetrafluorophenyl (TFP) ester of the terminal propionic acid group. The maleimido and TFP functional groups on the crosslinking compound sit at either end of a long, discrete-length chain of PEG (hence, dPEG®).

Crosslinking Reactions
One of the most popular, most useful crosslinking reactions in bioconjugate chemistry is the conjugation of free amines to free thiols. These reactions require heterobifunctional reagents that bridge the two groups. Typical crosslinkers are hydrophobic. Our dPEG® crosslinking products are water-soluble, amphiphilic, single molecular weight PEG compounds with discrete chain lengths.

Conventional hydrophobic crosslinking reagents frequently trigger aggregation and precipitation of the conjugates made from them. These problems do not happen with our water-soluble, non-immunogenic dPEG® crosslinkers.

Traditional PEGylation reagents are made from dispersed polymers. These polymeric reagents are intractably complex. This complexity creates difficulties in the analysis of conjugates. Our dPEG® products have no dispersity (Ð = 1). Each dPEG® product consists of a discrete chain of PEG with a single molecular weight. The uniform nature of our dPEG® products eliminates one of the most vexing analytical difficulties faced by users of traditional polymer PEG products.

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