MagQuant Plus DNA Kit V2

MagQuant Plus DNA Kit V2

Catalog Number:
NAEA531119MAG
Mfr. No.:
MQP-50096/50384/51920/55000/50096E/50384E/51920E
Price:
$812
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      • Overview
        • Magnetic bead based kit for normalization of DNA concentration, and quantitation of DNA for NGS and other applications.

          The MagQuant Plus DNA Kit V2 utilizes paramagnetic bead-based technology for genomic DNA quantitation and concentration normalization. The DNA normalization occurs during the purification process. Standard methods of DNA quantitation require the use of a standard curve derived from DNA samples of known concentrations; processing DNA samples from various sources would require extensive work and reagent use. MagQuant Plus DNA V2's proprietary technology allows to obtain a given DNA quantitation and required concentration of DNA samples regardless of the source by adding a specific number of beads to the DNA sample.

          Benefits
          · Rapid and reliable quantitation and normalization of DNA
          · No centrifugation step, no filtration step
          · Eliminates the need for standard curve
          · Allowing the processing of pool DNA samples from various sources
          · Equalizing concentration of genomic DNA input for DNA libray construction to help produce consistent and reliable NGS data without tedious initial input DNA quantitation.

          This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
          · Transfer DNA to be normalized to a 96-well PCR plate
          · Add Binding Buffer & MAG-C7 Beads
          · Bind appropriate amount of DNA to beads
          · Wash using Ethanol 2 times
          · Elution done using MB Elution Buffer
          · Store the DNA at -20°C.

          Please contact us at for specific academic pricing.

      • Properties
        • Other Properties
          Starting material (Sample matrix): DNA fragments, PCR products, and gDNA
          DNA input: Minimum DNA input for DNA fragments or genomic DNA normalization is 800 ng and for amplicon normalization is 300 ng.
          DNA Purity Requirements: A260/A230 should be around 1.7 or above
          Process method: Manual
          Purification method: Magnetic bead-based technology
          Elution Volume: 25-50μl (adjust as needed)

          * For research use only.

      • Applications
        • Application
          Next-Generation Sequencing (NGS)
          Application Description
          DNA quantitation and normalization of DNA concentration for:
          · Next Gen Sequencing library construction
          · Amplicon normalization
          · Genomic DNA normalization
          · PCR, qPCR

    Documents

    Protocol

    Handbook

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