-
-
Overview
-
Magnetic bead based kit for normalization of DNA concentration, and quantitation of DNA for NGS and other applications.
The MagQuant Plus DNA Kit V2 utilizes paramagnetic bead-based technology for genomic DNA quantitation and concentration normalization. The DNA normalization occurs during the purification process. Standard methods of DNA quantitation require the use of a standard curve derived from DNA samples of known concentrations; processing DNA samples from various sources would require extensive work and reagent use. MagQuant Plus DNA V2's proprietary technology allows to obtain a given DNA quantitation and required concentration of DNA samples regardless of the source by adding a specific number of beads to the DNA sample.
Benefits
· Rapid and reliable quantitation and normalization of DNA
· No centrifugation step, no filtration step
· Eliminates the need for standard curve
· Allowing the processing of pool DNA samples from various sources
· Equalizing concentration of genomic DNA input for DNA libray construction to help produce consistent and reliable NGS data without tedious initial input DNA quantitation.
This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
· Transfer DNA to be normalized to a 96-well PCR plate
· Add Binding Buffer & MAG-C7 Beads
· Bind appropriate amount of DNA to beads
· Wash using Ethanol 2 times
· Elution done using MB Elution Buffer
· Store the DNA at -20°C.Please contact us at for specific academic pricing.
-
- Properties
- Applications
-
Overview