LiveBox2 (LB2) Transparent Chamber for Cell Culture (two flow inlets and outlets)

LiveBox2 is transparent chamber, developed to model physiological barriers (e.g. lung and intestinal epithelium) in-vitro. LB2 is designed for inter-connected dynamic cell cultures and is equipped with two flow inlets and outlets, and a holder to house a porous membrane. The clamp system provided with the LB2 assures the watertight closure of the system in both static and dynamic conditions (up to 0.5mL/min in apical or basal compartments).
The removable transparent glass bottom allows for:
- Live imaging during static/dynamic culture, i.e. in situ imaging of the three-dimensional culture environment using optical microscopy.
- The removable holder system allows for:
- Housing of a porous membrane
- Easy and safe handling of the membrane where cells are seeded
- Post culture imaging including staining procedures or any other sample processing. After disassembling the LB2, the holder system supporting the cultivated cell monolayer or 3D construct can be easily removed from the bottom part and used for further investigations and analysis.
- Modularity: LB2 can be connected with other cell culture systems in order to mimic cross talk between tissues (multi organ simulation).
The LB2 kit is composed of:
- 1 LB2 equipped with glass discs
- 1 clamp systems
- PDMS tubes, luer lock connectors and 2 mixing chambers to realize two fluidic circuits
- 1 extractor

Please contact us at for specific academic pricing.