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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman RBPJAbout TargetGene symbol: RBPJ
Synonyms: AOS3 CBF1 csl IGKJRB IGKJRB1 KBF2 RBP-J RBPJK RBPSUH SUH
Species: HUMAN
Gene ID: 3516
Summary: The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_005349:5:4 NM_015874:4:4 NM_203283:4:4 NM_203284:5:4 NM_001363577:4:2 NM_001374400:5:4 NM_001374401:5:4 NM_001374402:5:4 NM_001374403:5:4
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