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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman RBFOX2About TargetGene symbol: RBFOX2
Synonyms: dJ106I20.3 Fox-2 FOX2 fxh HNRBP2 HRNBP2 RBM9 RTA
Species: HUMAN
Gene ID: 23543
Summary: This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_001031695:2:2 NM_001349982:3:3 NM_001349983:2:2 NM_001349989:3:3 NM_001349990:3:3 NM_001349991:3:3 NM_001349992:3:3 NM_001349994:3:3 NM_001349995:3:3 NM_001349996:3:3 NM_001349997:2:2 NM_001349998:2:2 NM_001349999:3:3 NM_014309:2:2 NM_001082576:2:2 NM_001082577:2:2 NM_001082578:3:3 NM_001082579:3:3
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- Properties
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Overview