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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman RALGAPA1About TargetGene symbol: RALGAPA1
Synonyms: GARNL1 GRIPE p240 RalGAPalpha1 TULIP1
Species: HUMAN
Gene ID: 253959
Summary: This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_014990:22:22 NM_194301:22:22 NM_001283043:23:23 NM_001283044:23:23 NM_001330075:22:22 NM_001346243:22:22 NM_001346245:23:23 NM_001346246:22:22 NM_001346247:23:23 NM_001346248:22:22 NM_001346249:23:23
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- Properties
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Overview