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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman ITSN2About TargetGene symbol: ITSN2
Synonyms: PRO2015 SH3D1B SH3P18 SWA SWAP
Species: HUMAN
Gene ID: 50618
Summary: This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. [provided by RefSeq, Jan 2017].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_006277:6:5 NM_147152:6:5 NM_019595:6:5 NM_001348181:7:4 NM_001348182:6:5 NM_001348183:6:5 NM_001348184:7:4 NM_001348185:6:5 NM_001348186:6:5
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- Properties
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Overview