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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman ICA1About TargetGene symbol: ICA1
Synonyms: ICA69 ICAp69
Species: HUMAN
Gene ID: 3382
Summary: This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2013].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_022307:6:5 NM_001276478:6:5 NM_001350819:6:5 NM_001350820:6:5 NM_001350821:6:5 NM_001350823:6:5 NM_001350824:6:5 NM_001350825:6:5 NM_001350826:6:5 NM_001350827:6:5 NM_001350828:6:5 NM_001350829:6:5 NM_001350830:6:5 NM_001350831:6:5 NM_001350832:6:5 NM_001350833:6:5 NM_001350834:6:5 NM_001350835:7:3 NM_001350836:7:3 NM_001350837:7:3 NM_001350838:6:5 NM_001136020:6:5 NM_004968:6:5
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- Properties
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Overview