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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman HS6ST1About TargetGene symbol: HS6ST1
Synonyms: HH15 HS6ST
Species: HUMAN
Gene ID: 9394
Summary: The protein encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biological activities. This enzyme is a type II integral membrane protein and is responsible for 6-O-sulfation of heparan sulfate. This enzyme does not share significant sequence similarity with other known sulfotransferases. A pseudogene located on chromosome 1 has been found for this gene. [provided by RefSeq, Jul 2008].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_004807:2:2
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