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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman HOTSAbout TargetGene symbol: HOTS
Synonyms: N/A
Species: HUMAN
Gene ID: 103344718
Summary: This gene encodes a protein that localizes to the nucleus and interacts with enhancer of rudimentary homolog protein. Overexpression of this protein can inhibit tumor growth. This gene is transcribed in antisense to the H19 non-coding transcript and is imprinted and maternally expressed. [provided by RefSeq, Nov 2015].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_001293171:1:1
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