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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman HK1About TargetGene symbol: HK1
Synonyms: hexokinase HK HK1-ta HK1-tb HK1-tc HKD HKI HMSNR HXK1 NEDVIBA RP79
Species: HUMAN
Gene ID: 3098
Summary: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_000188:3:3 NM_033496:3:3 NM_033497:6:4 NM_033498:6:4 NM_033500:7:3 NM_001322364:5:4 NM_001322365:8:5 NM_001322366:3:2 NM_001322367:3:3 NM_001358263:6:4
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