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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman HERC3About TargetGene symbol: HERC3
Synonyms: N/A
Species: HUMAN
Gene ID: 8916
Summary: This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_014606:8:6 NM_001271602:7:5 NM_001318505:8:6 NM_001375477:7:5 NM_001375478:8:6 NM_001375479:9:6 NM_001375480:8:6 NM_001375481:7:5 NM_001375482:8:6 NM_001375483:9:6
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- Properties
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