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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman HACE1About TargetGene symbol: HACE1
Synonyms: SPPRS
Species: HUMAN
Gene ID: 57531
Summary: This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm's tumor. [provided by RefSeq, Mar 2016].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_020771:12:12 NM_001350554:12:10 NM_001350555:9:9 NM_001350556:11:7 NM_001350557:10:6 NM_001350558:11:6 NM_001350559:10:6 NM_001350560:10:4 NM_001321080:11:11 NM_001321083:12:11 NM_001321084:11:6
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- Properties
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