Lentiviral human GJB2 sgRNA gene knockout kit

Lentiviral human GJB2 sgRNA gene knockout kit

Catalog Number:
CGKK543807FEN
Mfr. No.:
HKO-613503
Price:
$878
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      • Overview
        • Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers

          sgRNA lentiviral particle information
          • Functional titer: 1x10^8 TU/ml
          • Titration method: counting puromycin resistant cell clones
          • Purity: In vivo grade
          • Pseudotype: VSVG
          • Storage buffer: LentiShield™ lentivirus storage buffer
          • Storage: condition -80°C
          • Availability: 3-5 weeks

          sgRNA lentiviral plasmid information
          • Promoter for target sgRNA: U6
          • Mammalian selection: puromycin
          • Promoter for mammalian selection gene: EF1a
          • E.coli selection: Amp
          • Storage condition: -20°C
          • Availability: 1-2 weeks

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          Target Information

          Target Name
          Human GJB2
          About Target
          Gene symbol: GJB2
          Synonyms: CX26 DFNA3 DFNA3A DFNB1 DFNB1A HID KID NSRD1 PPK
          Species: HUMAN
          Gene ID: 2706
          Summary: This gene encodes a member of the gap junction protein family. The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene are responsible for as much as 50% of pre-lingual, recessive deafness. [provided by RefSeq, Oct 2008].
          NM numbers targeted (Accession number:Exon:CDS Fragment): NM_004004:2:1
      • Properties
        • * For Research Use Only.

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