-
-
Overview
-
Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman GFM1About TargetGene symbol: GFM1
Synonyms: COXPD1 EFG EFG1 EFGM EGF1 GFM hEFG1 mtEF-G1
Species: HUMAN
Gene ID: 85476
Summary: Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. [provided by RefSeq, Jul 2008].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_001308164:8:8 NM_001308166:7:7 NM_024996:7:7 NM_001374355:8:8 NM_001374356:6:6 NM_001374357:7:6 NM_001374358:4:4 NM_001374359:5:4 NM_001374360:5:4 NM_001374361:4:3
-
- Properties
-
Overview