Lentiviral human FN1 sgRNA gene knockout kit

Lentiviral human FN1 sgRNA gene knockout kit

Catalog Number:
CGKK544088FEN
Mfr. No.:
HKO-613222
Price:
$878
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      • Overview
        • Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers

          sgRNA lentiviral particle information
          • Functional titer: 1x10^8 TU/ml
          • Titration method: counting puromycin resistant cell clones
          • Purity: In vivo grade
          • Pseudotype: VSVG
          • Storage buffer: LentiShield™ lentivirus storage buffer
          • Storage: condition -80°C
          • Availability: 3-5 weeks

          sgRNA lentiviral plasmid information
          • Promoter for target sgRNA: U6
          • Mammalian selection: puromycin
          • Promoter for mammalian selection gene: EF1a
          • E.coli selection: Amp
          • Storage condition: -20°C
          • Availability: 1-2 weeks

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          Target Information

          Target Name
          Human FN1
          About Target
          Gene symbol: FN1
          Synonyms: CIG ED-B FINC FN FNZ GFND GFND2 LETS MSF SMDCF
          Species: HUMAN
          Gene ID: 2335
          Summary: This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016].
          NM numbers targeted (Accession number:Exon:CDS Fragment): NM_212482:10:10 NM_002026:10:10 NM_212478:10:10 NM_212476:10:10 NM_212474:10:10 NM_054034:10:10 NM_001306129:10:10 NM_001306130:10:10 NM_001306131:10:10 NM_001306132:10:10 NM_001365517:10:10 NM_001365518:10:10 NM_001365519:10:10 NM_001365520:10:10 NM_001365521:10:10 NM_001365522:10:10 NM_001365523:10:10 NM_001365524:10:10
      • Properties
        • * For Research Use Only.

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