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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman FN1About TargetGene symbol: FN1
Synonyms: CIG ED-B FINC FN FNZ GFND GFND2 LETS MSF SMDCF
Species: HUMAN
Gene ID: 2335
Summary: This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_212482:10:10 NM_002026:10:10 NM_212478:10:10 NM_212476:10:10 NM_212474:10:10 NM_054034:10:10 NM_001306129:10:10 NM_001306130:10:10 NM_001306131:10:10 NM_001306132:10:10 NM_001365517:10:10 NM_001365518:10:10 NM_001365519:10:10 NM_001365520:10:10 NM_001365521:10:10 NM_001365522:10:10 NM_001365523:10:10 NM_001365524:10:10
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Overview