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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman FGF1About TargetGene symbol: FGF1
Synonyms: AFGF ECGF ECGF-beta ECGFA ECGFB FGF-1 FGF-alpha FGFA GLIO703 HBGF-1 HBGF1
Species: HUMAN
Gene ID: 2246
Summary: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_000800:2:1 NM_033136:2:1 NM_033137:2:1 NM_001144892:2:1 NM_001144934:3:1 NM_001144935:2:1 NM_001257205:2:1 NM_001257206:2:1 NM_001257207:3:1 NM_001257208:2:1 NM_001257209:2:1 NM_001257210:2:1 NM_001257211:2:1 NM_001257212:2:1 NM_001354951:4:1 NM_001354952:3:1 NM_001354953:4:1 NM_001354954:3:1 NM_001354955:3:1 NM_001354956:3:1 NM_001354957:4:1 NM_001354958:3:1 NM_001354959:2:1 NM_001354961:4:1 NM_001354962:2:1 NM_001354963:3:1 NM_001354964:3:1
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