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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman DSTAbout TargetGene symbol: DST
Synonyms: BP240 BPA BPAG1 CATX-15 CATX15 D6S1101 DMH DT EBSB2 HSAN6 MACF2
Species: HUMAN
Gene ID: 667
Summary: This gene encodes a member of the plakin protein family of adhesion junction plaque proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the full-length nature of some variants has not been defined. It has been reported that some isoforms are expressed in neural and muscle tissue, anchoring neural intermediate filaments to the actin cytoskeleton, and some isoforms are expressed in epithelial tissue, anchoring keratin-containing intermediate filaments to hemidesmosomes. Consistent with the expression, mice defective for this gene show skin blistering and neurodegeneration. [provided by RefSeq, Mar 2010].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_015548:4:4 NM_001723:4:4 NM_183380:14:14 NM_001144769:17:17 NM_001144770:15:15 NM_001374736:18:18 NM_001374734:18:18 NM_001374722:18:18 NM_001374729:14:14 NM_001374730:14:14
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- Properties
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Overview