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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman DLG1About TargetGene symbol: DLG1
Synonyms: DLGH1 hdlg SAP-97 SAP97
Species: HUMAN
Gene ID: 1739
Summary: This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. A multitude of transcript variants deriving from alternative splicing and the use of multiple alternate promoter have been observed, including some splice variants that may be specific to brain and other tissues. An upstream uORF may regulate translation at some splice variants of this gene. [provided by RefSeq, Sep 2018].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_001098424:14:13 NM_004087:14:13 NM_001204386:13:12 NM_001204387:9:9 NM_001204388:9:9 NM_001290983:14:13 NM_001363865:13:12 NM_001366203:12:11 NM_001366204:13:12 NM_001366205:13:12 NM_001366206:12:11 NM_001366207:13:12 NM_001366208:13:12 NM_001366209:13:12 NM_001366210:13:12 NM_001366211:13:12 NM_001366212:13:12 NM_001366213:13:12 NM_001366214:14:13 NM_001366215:13:12 NM_001366216:12:11 NM_001366217:13:12 NM_001366218:14:13 NM_001366219:12:11 NM_001366220:12:11 NM_001366221:10:10 NM_001366222:10:10
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