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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman CNOT2About TargetGene symbol: CNOT2
Synonyms: CDC36 HSPC131 IDNADFS NOT2 NOT2H
Species: HUMAN
Gene ID: 4848
Summary: This gene encodes a subunit of the multi-component CCR4-NOT complex. The CCR4-NOT complex regulates mRNA synthesis and degradation and is also thought to be involved in mRNA splicing, transport and localization. The encoded protein interacts with histone deacetylases and functions as a repressor of polymerase II transcription. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_001199303:5:4 NM_001199302:6:4 NM_014515:5:4
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