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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman CACNA1GAbout TargetGene symbol: CACNA1G
Synonyms: Ca(V)T.1 Cav3.1 NBR13 SCA42 SCA42ND
Species: HUMAN
Gene ID: 8913
Summary: Voltage-sensitive calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division, and cell death. This gene encodes a T-type, low-voltage activated calcium channel. The T-type channels generate currents that are both transient, owing to fast inactivation, and tiny, owing to small conductance. T-type channels are thought to be involved in pacemaker activity, low-threshold calcium spikes, neuronal oscillations and resonance, and rebound burst firing. Many alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Sep 2011].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_018896:6:6 NM_198377:6:6 NM_198396:6:6 NM_198385:6:6 NM_198380:6:6 NM_198383:6:6 NM_198379:6:6 NM_198384:6:6 NM_198378:6:6 NM_198386:6:6 NM_198382:6:6 NM_198387:6:6 NM_198388:6:6 NM_198376:6:6 NM_001256324:6:6 NM_001256325:6:6 NM_001256326:6:6 NM_001256327:6:6 NM_001256328:6:6 NM_001256329:6:6 NM_001256330:6:6 NM_001256331:6:6 NM_001256332:6:6 NM_001256333:6:6 NM_001256334:6:6 NM_001256359:6:6 NM_001256360:6:6 NM_001256361:6:6
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- Properties
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Overview