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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman ARHGAP21About TargetGene symbol: ARHGAP21
Synonyms: ARHGAP10
Species: HUMAN
Gene ID: 57584
Summary: ARHGAP21 functions preferentially as a GTPase-activating protein (GAP) for CDC42 (MIM 116952) and regulates the ARP2/3 complex (MIM 604221) and F-actin dynamics at the Golgi through control of CDC42 activity (Dubois et al., 2005 [PubMed 15793564]).[supplied by OMIM, Mar 2008].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_020824:10:9 NM_001367448:10:9 NM_001367454:10:9 NM_001367447:9:8 NM_001367451:9:8 NM_001367453:9:8 NM_001367449:11:6 NM_001367450:12:6 NM_001367452:7:4 NM_001367455:3:2
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- Properties
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