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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman ADGRG1About TargetGene symbol: ADGRG1
Synonyms: BFPP BPPR GPR56 TM7LN4 TM7XN1
Species: HUMAN
Gene ID: 9289
Summary: This gene encodes a member of the G protein-coupled receptor family and regulates brain cortical patterning. The encoded protein binds specifically to transglutaminase 2, a component of tissue and tumor stroma implicated as an inhibitor of tumor progression. Mutations in this gene are associated with a brain malformation known as bilateral frontoparietal polymicrogyria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_005682:7:5 NM_001145770:7:5 NM_001145771:7:5 NM_001145772:7:5 NM_001145773:6:5 NM_001145774:7:5 NM_001290142:6:4 NM_001290143:6:3 NM_001290144:6:3 NM_201524:7:5 NM_201525:6:5 NM_001370428:7:5 NM_001370429:6:5 NM_001370430:6:5 NM_001370431:7:5 NM_001370432:6:5 NM_001370433:6:5 NM_001370434:7:5 NM_001370435:6:5 NM_001370436:7:5 NM_001370437:7:5 NM_001370438:6:5 NM_001370439:6:5 NM_001370440:7:5 NM_001370441:6:5 NM_001370442:7:5 NM_001370451:7:3 NM_001370453:7:3 NM_001370454:6:3
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- Properties
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Overview