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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman ADD1About TargetGene symbol: ADD1
Synonyms: ADDA
Species: HUMAN
Gene ID: 118
Summary: Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. [provided by RefSeq, Aug 2017].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_001119:9:8 NM_001354754:10:8 NM_001354755:9:8 NM_001354756:9:8 NM_001354757:10:8 NM_001354758:9:8 NM_001354759:9:8 NM_001354761:9:8 NM_001354762:9:8 NM_014189:9:8 NM_014190:9:8 NM_176801:9:8 NM_001286645:10:8
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