LACTB Recombinant Protein

LACTB Recombinant Protein

Catalog Number:
P001416692ABE
Mfr. No.:
Abe32-2490
Price:
  • Size:
    10 mg
    Quantity:
    Add to Cart:
      • Overview
        • Beta-Lactamase TEM precursor Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids and having a molecular mass of 29 kDa. Beta Lactamase is purified by proprietary chromatographic techniques. Beta-lactamase is a type of enzyme (EC 3.5.2.6) produced by some bacteria that is responsible for their resistance to beta-lactam antibiotics like penicillins, cephalosporins, cephamycins and carbapenems. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule''s antibacterial properties.

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      • Properties
        • Protein Name
          b-Lactamase, EC 3.5.2.6, TEM precursor.
          Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
          Formulation
          Lyophilized from a concentrated (1 mg/ml) solution in water containing 20mM Phosphate buffer pH-7.
          Storage
          Lyophilized Beta Lactamase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Beta Lactamase Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

      • Applications
        • Application Description
          It is recommended to reconstitute the lyophilized Beta Lactamase in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

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