L 012 sodium salt

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Background

L 012 sodium salt is a chemiluminescent probe [1].
L 012 sodium salt is a new chemiluminescent probe. In activated EoL-1 cells which generated reactive oxygen species (ROS), treatment with L-012 generated a significant chemiluminescence. While, superoxide dismutase (100-300 U/ml) abolished the chemiluminescence, which suggested that the main ROS detected was O2- [1]. In cultured endothelial cells, phorbol 12-myristate 13-acetate (PMA, 1 μM) caused an 2.8-fold increase of L-012 chemiluminescence. Vanadate (100 μM) increased the chemiluminescence up to 17-fold. These results suggested that the increase of superoxide produced by NAD(P)H oxidase [2].
In human oral cavity and blood and rat peritoneal cavity, L-012 reacted with ROS generated by activated neutrophils and generated strong chemiluminescence, which was higher than that of MCLA [3]. In nonobese diabetic mice and K8 knockout (K8(-/-)) mice, treatment with dextran sulphate sodium generated an increased L-012 chemiluminescence which detected reactive oxygen and nitrogen species (ROS and RNS) [4].

[1]. Nishinaka Y, Aramaki Y, Yoshida H, et al. A new sensitive chemiluminescence probe, L-012, for measuring the production of superoxide anion by cells. Biochem Biophys Res Commun, 1993, 193(2): 554-559.
[2]. Sohn HY, Gloe T, Keller M, et al. Sensitive superoxide detection in vascular cells by the new chemiluminescence dye L-012. J Vasc Res, 1999, 36(6): 456-464.
[3]. Imada I, Sato EF, Miyamoto M, et al. Analysis of reactive oxygen species generated by neutrophils using a chemiluminescence probe L-012. Anal Biochem. 1999, 271(1): 53-58.
[4]. Asghar MN, Emani R, Alam C, et al. In vivo imaging of reactive oxygen and nitrogen species in murine colitis. Inflamm Bowel Dis, 2014, 20(8): 1435-1447.