Kinase RR Kit for identification of protein kinase inhibitor

Identification of protein kinase inhibitor
Kinase RR Kit or Kinase Reaction rate Kit is developed for the easy and reliable identification of protein kinase inhibitor candidates from large compund libraries. The Kinase RR Kit uses direct bioluminescent measurement of ATP degradation rate to distingusih hits from non-hits. In contrast to other bioluminescent ATP based assays of kinase there is no interference from inhibitors acting on luciferase. Furthermore the standard curve is linerar rather than sigmodial. the Kinase RR Kit is flexible and can easily be adapted to various HTS strategies. Z' values up to 0.96 have been reported. The assay does not require radioactive materials, antibodies or any form of conjugates.

▶ Intrusments
It is recommended to use a microplate luminometer with a dispenser for adding ATP. However, microplate luminometers without dispensers can be used.

▶ Assay Procedure
Detailed described procedures of Standard Curve Generation, Substrate Optimisation, HTS and IC50 Determination are found in the "Instructions for Use", which is included with every kit.

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