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Schematic of polyUb chains enrichment

According to procedures described above, 5 mg whole cell lysates from HeLa cells were mixed with 0.2 mg GST (lane 1), GST-S5a (UIM) (lane 2), GST-TAB2 (NZF) (lane 3), GST-S5a (UIM) + GST-TAB2 (NZF) (lane 4), or GST-NEMO (UBAN) (lane 5). 10% eluates were separated by SDS-PAGE, followed by immunoblotting of Ub, K48-specific Ub chains and GST. * No immunoreactivity was detected in GST-NEMO (UBAN) pulldown assays, presumably because there is little linear polyubiquitin chains in cells.

K48 Polyubiquitin Chain Capture Kit

K48 Polyubiquitin Chain Capture Kit

Catalog Number:

UC01097301UBP

Mfr. No.:

J4430

Price:

$688

Size:

1 Kit

Quantity:

Add to Cart:

- Overview
  - This kit is designed for enrichment of cellular proteins conjugated with lysine 48-linked polyubiquitin chains using GST-S5a (UIM). Polyubiquitinated proteins bound on GST-S5a (UIM) can be precipitated using glutathione resin and eluted by a buffer containing 10 mM glutathione. Polyubiquitinated proteins can be assayed by immunoblotting or mass spectrometry.
    
    Please contact us at for specific academic pricing.
- Properties
  - Formulation
    
    Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
    
    Storage
    
    Store at -80°C; avoid multiple freeze-thaw cycles
    
    Concentration
    
    See tube label
    
    * Research Use Only

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