IDseek® Mitochondrial DNA HVR Sequencing by MPS Sample Kit

Intended use
Reverse Complement (RC-PCR) based MPS library prep solution for analysis of hypervariable regions (mutation hotspots) in very low quantities of (highly degraded) nuclear mtDNA in complex samples that do not provide a full STR. For laboratories in those countries where full genome sequencing in forensics is restricted by guidelines or regulations. RC-PCR provides multiplex targeted amplification, sequencing indexing and adaptor addition occuring simultaneously in a single closed tube reaction.

Compatibility
All amplicons are designed to meet the shortest possible fragment lengths and are compatible with illumina® MiSeq™ systems, with 2x 10 bp Unique Dual Index reads. IDseek® Kits have been tested with Tannic Acid and Humic Acid. RC-PCR technology allows the reliable analysis of highly degraded samples frequently found in forensic casework.

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