Human Intestinal Organoid-on-a-Chip Model

The human intestinal organs serve several functions including acting as a barrier, aiding in digestion and absorption, and facilitating movement. Moreover, they play a crucial role in regulating the physiological state of the entire body by metabolizing drugs and communicating with intestinal microorganisms as well as other organs.

• Recapitulation of physiological state of the human body
On the traditional Transwell, the cells only maintain a dense cobblestone structure. However, with the support of dynamic fluid, intestinal epithelial cells can now form a more complex 3D structure that closely resembles the human body. Additionally, the intestinal epithelial monolayer TEER gradually increases over time and remains stable after 10 days of operation. This makes it an excellent tool for pharmaceutical ADME studies.
• Characterization of Colon Organoid Differentiation Markers
Intestinal differentiation marker proteins Villin, Mucin 2 and glycocalyx were expressed and distributed on the apical side of the colon.
• Stronger intestinal absorption
Under fluid stimulation, the P-gp transporter has a stronger function. After culturing for 24 days, fluorescence imaging was performed with the presence of a P-gp inhibitor. A strong fluorescence accumulation of Rhodamine was observed in the cells.

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