Name: HRP
Price: Inquiry

- Overview
  - HRP consists of the basic isoenzyme having a molecular weight of 44 kDa. The Horseradish Peroxidase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity. The enzyme horseradish peroxidase, found in horseradish, is used extensively in molecular biologyand in antibody amplification and detection, among other things. For example, In recent years the technique of marking neurons with the enzyme horseradish peroxidase (HRP) has become a major tool. In its brief history, this method has probably been used by more neurobiologists than have used the Golgi stainsince its discovery in 1870. Horseradish peroxidase is also highly used in techniques such as Western blottingand ELISAs. HRP is widely used as an enzymatic label in immunoassays. Usually, the enzyme is coupled to antibodies, lectins or haptens. Coupling to antibodies etc. may be performed through the carbohydrate side chains of the HRP.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    Horseradish Peroxidase, HRP, EC 1.11.1.7.
    
    Source
    
    Root extracts of horseradish
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    Greater than 268 U/mg (25°C, guaiacol as the hydrogen donor, pH-7 and H2O2 as substrates).
    
    Formulation
    
    HRP consists of the basic isoenzyme having a molecular weight of 44 kDa. The Horseradish Peroxidase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.
    
    Storage
    
    Lyophilized HRP although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HRP should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application Description
    
    It is recommended to reconstitute the lyophilized HRP in sterile 18MΩ-cm H2O not less than 100 μg/ml or more than 10 mg/ml solutions. Greater than 268 U/mg (25°C, guaiacol as the hydrogen donor, pH-7 and H2O2 as substrates).

HRP

HRP

We Also Recommend

HS3ST1 Recombinant Protein

HSD17B1 Recombinant Protein

HSD17B11 Recombinant Protein