HIV-1 (BG505) Luciferase Pseudotyped Virus

The HIV-1 (BG505) Luciferase Pseudotyped Virus is a replication-defective virus produced by expressing the structural proteins (gag-pol) and envelope glycoprotein (Env) of Human Immunodeficiency Virus Type 1 (HIV-1) in HEK-293T cells. The virus self-assembles and encapsulates the nucleic acid expressing the luciferase reporter gene. Since this pseudovirus cannot produce new viral particles after infection, its safety is high, and its structure closely resembles that of the actual virus. Therefore, it can be used for neutralizing antibody titer determination, inhibitor screening, viral invasion studies, and HIV-1 (BG505) vaccine development.

The HIV-1 pseudovirus contains the virus's neutralizing antigens and is capable of infecting cells. When a sample has neutralizing activity, its ability to infect cells will be reduced after interacting with the pseudovirus. By measuring the luciferase expression in each well using a multifunctional microplate reader, the sample well's values can be compared with the pseudovirus control group to determine whether the sample has neutralizing activity and calculate its effective concentration. The neutralizing antibody titer refers to the reciprocal of the dilution factor of the antibody at which 50% of the pseudovirus is inhibited.

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