HighPrep PCR

HighPrep PCR is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of amplicons and tight size-specific selection of DNA fragments. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapter ligation, microarrays, and so on. This protocol can be used for manual procedures as well as in automation.

Benefits
· Rapid and reliable post-PCR and DNA clean-up reagent
· Achieve uniform and consistent DNA fragments
· High recovery of amplicons >100 bp
· Uniform fragments size distribution
· Adaptable to high throughput liquid handling workstations

This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
Add HighPrep PCR reagent to the reaction mixture
Bind DNA to paramagnetic beads
Separation of beads and DNR from contaminants
Wash beads with Ethanol to remove contaminants
Elute purified PCR products or other DNA fragments from beads

Please contact us at for specific academic pricing.